Journal of translational medicine
Adult, Aged, Caveolin 1 -- metabolism, Female, Humans, Lymphatic Metastasis, Metabolic Networks and Pathways -- physiology, Middle Aged, Neoplasm Invasiveness, Prognosis
Inflammatory breast cancer (IBC) is the most aggressive form of breast cancer. In non-IBC, the cysteine protease cathepsin B (CTSB) is known to be involved in cancer progression and invasion; however, very little is known about its role in IBC. In this study, we enrolled 23 IBC and 27 non-IBC patients. All patient tissues used for analysis were from untreated patients. Using immunohistochemistry and immunoblotting, we assessed the levels of expression of CTSB in IBC versus non-IBC patient tissues. Previously, we found that CTSB is localized to caveolar membrane microdomains in cancer cell lines including IBC, and therefore, we also examined the expression of caveolin-1 (cav-1), a structural protein of caveolae in IBC versus non-IBC tissues. In addition, we tested the correlation between the expression of CTSB and cav-1 and the number of positive metastatic lymph nodes in both patient groups. Our results revealed that CTSB and cav-1 were overexpressed in IBC as compared to non-IBC tissues. Moreover, there was a significant positive correlation between the expression of CTSB and the number of positive metastatic lymph nodes in IBC. CTSB may initiate proteolytic pathways crucial for IBC invasion. Thus, our data demonstrate that CTSB may be a potential prognostic marker for lymph node metastasis in IBC.
Mohamed, Nouh A.; Mohamed, Mona M.; El-Shinawi, Mohamed; Shaalan, Mohamed A.; Cavallo-Medved, Dora; Khaled, Hussein M.; and Sloane, Bonnie F., "Cathepsin B: a potential prognostic marker for inflammatory breast cancer." (2011). Journal of translational medicine, 9, 1.