Title

Derivation of a Novel G2 Reporter System

Document Type

Article

Publication Date

2014

Publication Title

Cytotechnology

DOI

10.1007/s10616-014-9757-5

Keywords

Cell cycle, G2 phase, Mitosis, DNAsynthesis, Cyclin B1

Abstract

Abstract Progression through G2 phase of the cell cycle is a technically difficult area of cell biology to study due to the lack of physical markers specific to this phase. The FUCCI system uses the biology of the cell cycle to drive fluorescence in select phases of the cell cycle. Similarly, a commercially available system has used a fluorescent analog of the Cyclin B1 protein to visualize cells from late S phase to the metaphase– anaphase transition. We have modified these systems to use the promoter and destruction box elements of Cyclin B1 to drive a cyan fluorescent protein. We demonstrate here that this is a useful tool for measuring the length of G2 phase without perturbing any aspect of cell cycle progression.