Title

Electrospray interfacing for the coupling of ion-exchange and ion-paring chromatography to mass spectrometry

Document Type

Article

Publication Date

1991

Publication Title

Journal of Chromatography A

Volume

554

Issue

2

First Page

27

Last Page

38

Keywords

conference paper, ion exchange chromatography, ion pair chromatography, mass spectrometry, priority journal

Abstract

Electrospray was evaluated as an interface for coupling ion-exchange and ion-pairing chromatography to mass spectrometry. Effective separation and good sensitivity were demonstrated for a liquid flowrate as high as 50 μl/min and a matrix ion concentration as high as 10 mM. Minimum detectable amounts were estimated to be five to ten times poorer than those obtained in the absence of ion-exchange buffers or ion-pairing reagents. The minimum detectable amount for arsenobetaine after a dynamic ion-exchange separation was estimated to be about 20 pg. © 1991.