Title

Ion-spray mass spectrometry of marine neurotoxins.

Document Type

Article

Publication Date

1989

Publication Title

Rapid communications in mass spectrometry : RCM

Volume

3

Issue

5

First Page

145

Last Page

150

Keywords

domoic acid, drug derivative, kainic acid, marine toxin, neurotoxin, saxitoxin, tetrodotoxin, article, mass spectrometry, methodology, Kainic Acid, Marine Toxins, Neurotoxins, Saxitoxin, Spectrum Analysis, Mass, Tetrodotoxin

Abstract

Ion-spray mass spectrometry was investigated for the analysis of three marine neurotoxins: domoic acid, saxitoxin and tetrodotoxin. All three compounds gave positive-ion spectra with abundant ions of protonated molecules and no significant fragmentation. Domoic acid gave a negative-ion spectrum with a strong [M-H]- ion. Tandem mass spectrometry provided useful fragment-ion spectra for all compounds. Detection limits for flow injection analyses with selected-ion monitoring were determined to be 30 pg for saxitoxin, 100 pg for domoic acid and 200 pg for tetrodotoxin. Combining liquid chromatography with ion-spray mass spectrometry allowed the determination of domoic acid and some of its isomers in toxic shellfish tissue extracts.