Title

Characterization of a novel human CAP2 interacting protein: Neuronal calcium binding protein 1 (Necab1).

Date of Award

2003

Degree Type

Thesis

Degree Name

M.Sc.

Department

Biological Sciences

First Advisor

Hubberstey, A.

Keywords

Chemistry, Biochemistry.

Rights

CC BY-NC-ND 4.0

Abstract

The actin cytoskeleton is implicated in numerous processes such as cellular division, migration, endocytosislexocytosis, and more recently synaptic plasticity. Important players in controlling cytoskeletal rearrangements are the Cyclase Associated Proteins (CAPs) which are thought to link cell signalling to the dynamics of the actin cytoskeleton, via sequestration of monomeric actin subunits. CAPs are conserved throughout evolution and possess conserved functional domains. Previous studies have suggested a link between CAP proteins and vesicle trafficking and this study focussed on characterizing binding partners of human CAPs. Using a two hybrid screen of a human adult brain cDNA library, a novel human CAP interacting protein named Neuronal calcium binding protein 1 (Necab1) has been isolated. Necab1 is a 351 amino acid protein containing a predicted calcium binding EF-hand and three coiled-coil domains. It is highly expressed in human brain, and similar to CAP2, displays high levels of expression within the hippocampus and cerebral cortex of rat brain. In vitro binding and in vivo immunoprecipitations demonstrate that CAP2 interacts with Necab1. (Abstract shortened by UMI.)Dept. of Biological Sciences. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis2002 .M68. Source: Masters Abstracts International, Volume: 43-01, page: 0215. Adviser: A. V. Hubberstey. Thesis (M.Sc.)--University of Windsor (Canada), 2003.