Title

Characterization of the gene encoding cathepsin L-associated protein from Artemia franciscana and its expression during development.

Date of Award

2005

Degree Type

Thesis

Degree Name

M.Sc.

Department

Biological Sciences

Keywords

Biology, Molecular.

Rights

CC BY-NC-ND 4.0

Abstract

The major cysteine protease in embryos and larvae of the brine shrimp, Artemia franciscana, is a heterodimeric protein consisting of a catalytic subunit (28.5 kDa) with a high degree of homology with cathepsin L (CL), and a non-catalytic subunit (31.5 kDa), cathepsin L-associated protein (CLAP). My study mainly focuses on identification and characterization of the CLAP cDNA and its gene. An Artemia franciscana cDNA library was screened to identify the full-length cDNA of the CLAP. Sequence analysis of positive cDNA clones revealed the presence of at least two variants of the CLAP gene, one has 1888 bp with an open reading frame (ORF) of 996 bp coding for 332 amino acids, and the other has 1870 bp with an ORF of 945 bp coding for 315 amino acids. This is the first study of a cathepsin L-associated protein with well defined cell adhesion domains. The DNA sequence of two CLAP cDNAs and the CLAP gene can be found in the NCBI database with accession numbers AY307377, AY462276 and AY757920, respectively. (Abstract shortened by UMI.)Dept. of Biological Sciences. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis2005 .L585. Source: Masters Abstracts International, Volume: 44-01, page: 0263. Thesis (M.Sc.)--University of Windsor (Canada), 2005.