Date of Award


Degree Type


Degree Name



Civil and Environmental Engineering

First Advisor

Biswas, N.


Engineering, Civil.




Arthromyces ramosus peroxidase (ARP) has been studied extensively to catalyze the oxidation of various phenolic compounds in the presence of hydrogen peroxide to form polymers which are readily precipitated from solution. The objective of this thesis was to extend research to other industrial wastewaters. Azo dyes and one representative dye breakdown product: 1,4-phenylenediamine (p-PD) were chosen. Experiments were conducted to investigate the feasibility and efficiency of using ARP and hydrogen peroxide to reduce the color of the azo dyes and to remove 1,4-phenylenediamine (p-PD). All tests were carried out in continuously-stirred batch reactors. The dyes selected included Naphthol Blue Black, Crocein Orange G, Acid Red 4, and Disperse Orange 3. The optimum conditions to achieve highest efficiency of dye color reduction or p-PD removal were determined for the following parameters: pH, ARP concentration, hydrogen peroxide concentration and reaction time. ARP efficiently reduced the color of the selected dyes except Disperse Orange 3 from synthetic wastewater in the presence of hydrogen peroxide. The optimum pH for different dyes was 7.0--8.0. The optimum ARP concentration range was from 0.1 to 0.2 U/mL, the optimum H2O2 range was from 100--200 muM for 50 muM dyes and the reaction time needed was less than 1 hour. Experiments were also conducted to reduce the color of the Disperse Orange 3 using zero-valent iron. Results showed that more than 98% conversion efficiency could be achieved in 1 hour. For p-PD removal, the optimum pH was 7.6 and the optimum ARP concentration, hydrogen peroxide concentration, and reaction time were 0.02 U/mL, 120 muM, 4 h for 100 muM p-PD and 0.05 U/mL, 400 muM and 4 h for 300 muM p-PD. A simple and convenient colorimetric method was developed to measure 1,2-phenylenediamine (o-PD), 1,3-phenylenediamine (m-PD), 1,4-phenylenediamine (p-PD) and aniline based on their reaction with trinitrobezenesulfonic acid (TNBS) in the presence of sodium sulfite.Dept. of Civil and Environmental Engineering. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis2002 .W36. Source: Masters Abstracts International, Volume: 41-04, page: 1147. Advisers: N. Biswas; J. K. Bewtra. Thesis (M.A.Sc.)--University of Windsor (Canada), 2002.