Date of Award

1992

Degree Type

Thesis

Degree Name

M.Sc.

Department

Chemistry and Biochemistry

Keywords

Chemistry, Biochemistry.

Rights

CC BY-NC-ND 4.0

Abstract

Growth of P. putida using gluconate as a carbon source effects a considerable decrease in the rate of 4-deoxy-4-fluoro-D-glucose (4FG) metabolism to 2,3-dideoxy-D-glycero-pentonic acid (2,3-DDRA). In vivo $\sp{19}$F-NMR spectroscopic analyses of 4FG metabolism indicate that fluoride release is the initial step preceeding 4FG metabolism to 2,3-DDRA. Crude periplasmic extracts produced by osmotic shock lack fluoride release activity. This suggests that defluorination is not dependent upon the initial binding of a periplasmic protein to 4FG. The binding protein of P. putida lacks a binding affinity for 4FG as indicated by the absence of significant chemical shifting observed by $\sp{19}$F-NMR in incubations of 4FG with periplasmic extracts. Cytoplasmic membrane preparations exhibit significant fluoride release activity only in the presence of the electron donors L-malate/FAD and phenazine methosulphate/ascorbate. In the presence of electron donors approximately 20-25% of the initial fluoride substituent of 4FG is released as fluoride ion. Glucose elicits a total inhibition of fluoride release indicating the greater affinity of the defluorinating protein for glucose than 4FG. (Abstract shortened by UMI.)Dept. of Chemistry and Biochemistry. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1992 .T454. Source: Masters Abstracts International, Volume: 31-04, page: 1798. Thesis (M.Sc.)--University of Windsor (Canada), 1992.

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