Title

Examining the effect of Plk4/Sak levels on the transcript profiles of other genes

Date of Award

2008

Degree Type

Thesis

Degree Name

M.Sc.

Department

Biological Sciences

First Advisor

Hudson, John (Biological Sciences)

Keywords

Biology, Molecular.

Rights

CC BY-NC-ND 4.0

Abstract

The aberrant regulation of cell cycle checkpoints can potentially initiate cellular transformation to an oncogenic state that leads to tumor formation. Many families of cell cycle regulators are present to ensure that normal cellular growth and replication occurs, including the polo-like kinases (Plk). Plk4 (Sak), the newest and most structurally divergent member of the Plks has been implicated to play crucial roles in centrosome dynamics and mitotic progression. Plk4 heterozygous mouse embryonic fibroblasts (MEFs) present a number of phenotypic differences, in comparison to their wild type counterparts that may contribute to the increased incidence of tumor formation observed in heterozygous Plk4 mice. Microarray technology was employed to investigate transcriptional differences between the wild type and heterozygous Plk4 MEFs. Furthermore, transcriptional and protein differences were examined in the Plk4 MEFs in response to DNA damaging agents, to explore a possible role for Plk4 in the DNA damage pathways.