Date of Award

1994

Degree Type

Dissertation

Degree Name

Ph.D.

Department

Chemistry and Biochemistry

First Advisor

Adeli, K.

Keywords

Chemistry, Biochemistry.

Rights

CC BY-NC-ND 4.0

Abstract

Faulty regulation of various apolipoproteins by the human liver may be an important contributor in the development of coronary heart disease. The factors that regulate hepatic apolipoprotein production are largely unknown. In cell culture, insulin appeared to reduce the secretion of apolipoprotein B, whereas thyroid hormone stimulated apolipoprotein B secretion. Both of these effects were found to be dose-dependent. In order to understand the mechanisms underlying the regulation of hepatic apolipoprotein B secretion, a cell-free translation system derived from a human hepatoma cell-line, HepG2, was developed to investigate the rate of apolipoprotein B synthesis. Extracts of HepG2 cells were found to have high in vitro protein synthesizing activity, and were shown to efficiently synthesize in vitro a number of liver specific proteins, including the unusually large apolipoprotein B molecule. This in vitro system along with whole-cell pulse labeling experiments were used to study the effects of insulin and thyroid hormone on apolipoproteins B, E and A-I synthesis and secretion in HepG2 cells. Source: Dissertation Abstracts International, Volume: 56-01, Section: B, page: 0226. Adviser: Khosrow Adeli. Thesis (Ph.D.)--University of Windsor (Canada), 1994.

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