Title

I. THE DEVELOPMENT OF TWO ASSAYS, EMPLOYING ENZYMES AS REAGENTS, FOR THE DETERMINATION OF LECITHIN AND TOTAL CHOLINE-CONTAINING PHOSPHOLIPIDS IN AMNIOTIC FLUID. II. A STUDY OF CO-SUBSTRATES FOR PEROXIDASE COUPLED REACTIONS. III. A STUDY OF THE APPLICABILITY OF A BIURET PROCEDURE AND TWO DYE-BINDING PROCEDURES TO TOTAL PROTEIN AND ALBUMIN DETERMINATIONS IN CEREBROSPINAL FLUID.

Date of Award

1980

Degree Type

Dissertation

Degree Name

Ph.D.

Department

Chemistry and Biochemistry

Keywords

Chemistry, Biochemistry.

Rights

CC BY-NC-ND 4.0

Abstract

Part I. Two assays, one for the determination of lecithin, the other for total choline-containing phospholipids in amniotic fluid, which employ enzymes as reagents have been developed, optimized and carried through the initial clinical trials. Unlike previous procedures for the prediction of respiratory distress syndrome, both assays are relatively quick, simple, inexpensive, involve no extractions, and are amenable to automation. Of even greater significance is that both are quantitative and apparently unaffected by hemoglobin, cellular debris and bilirubin, common interferences in previous procedures. Part II. A study of co-substrates for peroxidase catalyzed color reactions has been conducted. These substrates were compared on the basis of sensitivity, initial rate, stability of the chromogen, time required for maximum color development, and degree of blank reaction. Observations have been made as to the basic structural requirements necessary to fulfill the requirements as dictated by the above considerations. Part III. A study of the applicability of a biuret procedure for total protein determinations as well as bromcresol green and bromcresol purple procedures for albumin determinations in cerebrospinal fluid (Csf) has been conducted. Particular attention has been given to the effects of low molecular weight species, present in Csf on the three assays. Filtration through ultrafiltration membrane cones was found to be a relatively quick and simple way to separate low molecular weight material from larger proteins. Source: Dissertation Abstracts International, Volume: 41-03, Section: B, page: 0922. Thesis (Ph.D.)--University of Windsor (Canada), 1980.