Date of Award

1991

Publication Type

Master Thesis

Degree Name

M.Sc.

Department

Biological Sciences

Keywords

Chemistry, Biochemistry.

Rights

info:eu-repo/semantics/openAccess

Abstract

An immunodetection assay on Western blots has been used to determine the thiol proteinase content and composition in cysts from twelve populations of the brine shrimp, Artemia. Our results showed no differences in the subunit composition of the thiol proteinase among cysts from eight bisexual strains and four parthenogenic strains. In contrast, we found that Artemia cysts from parthenogenic strains contain 17.1 ng/cyst of the thiol proteinase, while cysts from bisexual strains contain 8.2 ng/cyst of the thiol proteinase. In order to do further study on the thiol proteinase of Artemia, a cDNA library of Artemia embryos was screened and the positive cDNA colonies coding for the thiol proteinases were isolated. Two positive signal colonies were selected by direct immunological screening method, but no significant amounts of recombinant phagemid DNA could be isolated from these clones. When the cDNA library was re-screened using a $\sp{32}$P-labeled oligonucleotide probe, one colony (TPa) was selected. Large amounts of phagemid DNA could be easily obtained and the inserted DNA was 1.8 Kb in length. Calculations suggest that the cloned DNA can encode for a protein of 50-60 kDa which is in the molecular weight range of the two subunits (combined) of the Artemia thiol proteinase. (Abstract shortened by UMI.)Dept. of Biological Sciences. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1991 .L855. Source: Masters Abstracts International, Volume: 30-04, page: 1336. Thesis (M.Sc.)--University of Windsor (Canada), 1991.

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