Date of Award
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An in vivo sister chromatid exchange (SCE) assay system for monitoring environmental genotoxicity, using bone marrow cells has been developed, tested and applied to groups of inbred and wild mice under various environmental conditions. The best results were obtained with nine, serial, intraperitoneal injections of 5-bromo-2'-deoxyuridine (BUdR) (40 (mu)g/g of body weight) and 5-fluorodeoxyuridine (FUdR) (2 (mu)g/g of b.w.) and then with 5 (mu)g/g of b.w. of colchicine, given on the 27th hour following the first injections of BUdR/FUdR. The mice were sacrificed 3 hours later. With the above injection schedule, male mice of C3H/HeJ and C57BL/6J maintained in the laboratory gave a mean (+OR-) SEM baseline SCE value of 3.42 (+OR-) 0.07 and 3.62 (+OR-) 0.08 respectively. The female mice of the same strains gave higher numbers of SCEs/cell. Males obtained from natural populations in southwestern Ontario had a higher mean SCE value (6.02 (+OR-) 0.16) as did the inbred males maintained in outdoor enclosures (5.08 (+OR-) 0.22). Wild mice maintained in the laboratory for a period of six months or more, gave SCE values similar to those of the control mice (3.46 (+OR-) 0.12). The SCE values in wild caught mice and in enclosure maintained inbreds were inversely proportional (r = -0.49) to the distance between the sites where the animals were either collected or maintained and the nearest major industrial centres. Based on the differences in SCE values between laboratory maintained mice and wild mice as well as laboratory maintained and inbred mice housed at various outdoor sites together with the geographic patterns in SCE levels, it is suggested that the in vivo SCE analysis in bone marrow cells using the bromodeoxyuridine substitution method, has the potential of being an early warning surveillance system for the general levels of environmental genotoxic agents.Dept. of Biological Sciences. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1983 .N393. Source: Dissertation Abstracts International, Volume: 44-03, Section: B, page: 0679. Thesis (Ph.D.)--University of Windsor (Canada), 1983.
NAYAK, BICHITRA NANDA., "IN VIVO SISTER CHROMATID EXCHANGES: A TEST SYSTEM MONITORING ENVIRONMENTAL GENOTOXICITY." (1983). Electronic Theses and Dissertations. 2414.