Date of Award
1987
Publication Type
Doctoral Thesis
Degree Name
Ph.D.
Department
Chemistry and Biochemistry
Keywords
Chemistry, Biochemistry.
Rights
info:eu-repo/semantics/openAccess
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Abstract
A technique has been developed to determine ATP and pyrophosphate from one aliquot of platelets. The sample which contains both ATP and pyrophosphate was used to generate NAD$\sp{+}$ through a sequence of enzymatic reactions starting with ATP. The NAD$\sp{+}$ generated was condensed with acetone to form a fluorescent product. Prior to the formation of this product, pyrophosphate was separated from NAD$\sp{+}$ by passage of the reaction mixture through a Dowex-1 (Cl$\sp{-}$) resin and subsequent elution with HCl. Recoveries for both assays ranged from 98-106%, and coefficients of variation for both within-run and between-run studies for both assays were below 7%.Dept. of Chemistry and Biochemistry. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1987 .S387. Source: Dissertation Abstracts International, Volume: 48-10, Section: B, page: 2962. Thesis (Ph.D.)--University of Windsor (Canada), 1987.
Recommended Citation
SEUDEAL, NARACE DYAL., "NEW FLUOROMETRIC METHODS FOR THE DETERMINATION OF ALUMINUM, PYROPHOSPHATE, AND ADENOSINE 5'-TRIPHOSPHATE IN BIOLOGICAL SYSTEMS." (1987). Electronic Theses and Dissertations. 2841.
https://scholar.uwindsor.ca/etd/2841