Title

Role of mitochondria in neuronal cell death induced by oxidative stress: Neuroprotection by coenzyme Q(10).

Date of Award

2004

Degree Type

Thesis

Degree Name

M.Sc.

Department

Chemistry and Biochemistry

First Advisor

Pandey, S.,

Keywords

Chemistry, Biochemistry.

Rights

CC BY-NC-ND 4.0

Abstract

In past research, oxidative stress has been implicated in aging and age related disorders. Cell death caused by oxidative stress has been shown to play an important role in neuro-degenerative diseases. We used differentiated Human Neuroblastoma (SH-SY5Y) and Teratocarcinoma (NT2N) cells as models to study the mechanism of cell death induced by oxidative stress. We observed that differentiated NT2N AND SH-SY5Y cells underwent apoptosis following oxidative stress induced by direct hydrogen peroxide treatment. Morphological, apoptotic features including nuclear condensation and membrane blebbing and biochemical changes including DNA fragmentation and caspase and proteasome activation were evident following oxidative stress. We further investigated the production of reactive oxygen species and mitochondrial dysfunction in the cell under oxidative stress. There was an increase in total ROS produced by the cells after H2O2 treatment. Furthermore, there was a decrease in the mitochondrial membrane potential and an increase in mitochondrial ROS generation. (Abstract shortened by UMI.)Dept. of Chemistry and Biochemistry. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis2004 .S66. Source: Masters Abstracts International, Volume: 43-01, page: 0216. Adviser: S. Pandey. Thesis (M.Sc.)--University of Windsor (Canada), 2004.