Title

Role of Xenopus laevis integrin linked kinase (XILK) during early development.

Date of Award

2005

Degree Type

Thesis

Degree Name

M.Sc.

Department

Biological Sciences

Keywords

Biology, Molecular.

Rights

CC BY-NC-ND 4.0

Abstract

Integrin linked kinase (ILK) is a serine/threonine protein kinase implicated in the phosphatidylinositol 3'kinase (PI3'K) pathway (Delcommenne et al., 1998). In our study, we isolated and characterized a cDNA clone encoding ILK in Xenopus laevis (X-ILK). The experiments were executed in both embryos and adult tissues to compare the relationship between the ILK expression patterns. Sequence analysis of X-ILK revealed that it is 59% identical to human ILK (HILK) cDNA and 71% identical to human ILK (H-ILK) protein. The well-known domains of ILK (ankyrin domain, Pleckstrin homology domain, kinase domain and paxillin binding site) are preserved among human, mouse, rat and Drosophila. These domains are found in Xenopus ILK. Northern blot analysis showed that a 1.8 Kb transcript is present throughout early embryogenesis. However, there was a significant increase in X-ILK expression at the onset of neurulation. Interestingly, expression studies revealed the presence of only one transcript whereas Western blot analysis revealed the expression of two X-ILK proteins during early development. (Abstract shortened by UMI.) Source: Masters Abstracts International, Volume: 44-03, page: 1295. Thesis (M.Sc.)--University of Windsor (Canada), 2005.