Title

Some aspects of the cell biology of lipid S-nitrosothiols.

Date of Award

2005

Degree Type

Thesis

Degree Name

M.Sc.

Department

Chemistry and Biochemistry

Keywords

Chemistry, Biochemistry.

Rights

CC BY-NC-ND 4.0

Abstract

Nitric Oxide is an important messenger and mediator in biological systems. Due to its short half life, S-nitrosothiols (RSNOs) have been suggested to be one of the important biomolecules for the storage and transport of NO. RSNOs also exhibit NO-like bioactivities. In the present study, we focus on the study of some aspects of cell biology of lipid S-nitrosothiols. In the first part of the study, we focus on the comparison of the cellular uptake of native LDL and chemically modified (homocysteinylation and S-nitrosation) LDL by Normal Human Fibroblasts (NHFs). Here, S-nitroso homocysteinylated LDL (LDL-SNO) was synthesized through the reaction of homocysteinylated LDL (LDL-SH) and S-nitrosocysteine. In the second part of the study, we focus on the comparison of the role of lipid S-nitrosothiols in apoptosis under light and dark conditions. Here, S-nitroso-octadecanethiol, a novel and lipophilic S-nitrosothiol, was synthesized to explore its apoptotic role in the SK-MEL-28 human melanoma cells under dark and light conditions. The present study demonstrated that S-nitroso-octadecanethiol could induce apoptosis in SK-MEL-28 human melanoma cells. (Abstract shortened by UMI.) Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis2005 .W363. Source: Masters Abstracts International, Volume: 44-03, page: 1371. Thesis (M.Sc.)--University of Windsor (Canada), 2005.