Date of Award

1998

Degree Type

Thesis

Degree Name

M.Sc.

Department

Biological Sciences

First Advisor

Zielinski, B. S.,

Keywords

Biology, Neuroscience.

Rights

CC BY-NC-ND 4.0

Abstract

Neurogenesis has traditionally been viewed as an embryonically restricted event with few exceptions. The peripheral olfactory organ and the olfactory bulb are two regions that are exceptions to the rule. This is the first study to investigate cell proliferation simultaneously in the peripheral olfactory organ and the brain of the 1--2 year age class larval sea lamprey, Petromyzon marinus. Cell proliferation was assessed using the thymidine analogue, 5-bromo-2'-deoxyuridine (BrdU). The proliferative density over 14 days was similar in the olfactory epithelium. However, the proliferative density was higher in the dorsal respiratory epithelium than in the dorsal olfactory epithelium. Within the olfactory epithelium, BrdU labeled cells were prominent in three zones: in the lateral region adjacent to the respiratory epithelium, in the caudal lateral region, and in the septum. BrdU labeled cells migrated from the base of the epithelium to the nuclear layer by day 14. Cell proliferation was also noted in the lamella troughs of stage 7 metamorphic lampreys. In the olfactory bulb, BrdU labeled cells were predominant in the ependymal layer at day 1. By day 14, some of the labeled cells migrated into the granule cell and glomerular layers. In small lampreys (5--10 cm in length), a group of ORN afferents, labeled with GS-1 lectin was seen projecting from the glomerular layer to the ependymal layer where BrdU labeled cells were observed. This suggests that the afferent fibers could modulate neurogenesis in the larval lamprey. Cell proliferation was also prevalent in the habenula, with the right side showing more BrdU labeled cells than the left. A comparison between lampreys that have acclimated to laboratory conditions and those recently retrieved from the natural habitat showed that cell proliferation was more conspicuous in the lampreys from the natural habitat.Dept. of Biological Sciences. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1998 .H83. Source: Masters Abstracts International, Volume: 39-02, page: 0454. Adviser: B. S. Zielinski. Thesis (M.Sc.)--University of Windsor (Canada), 1998.

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