Date of Award

1983

Degree Type

Dissertation

Degree Name

Ph.D.

Department

Chemistry and Biochemistry

Keywords

Chemistry, Biochemistry.

Rights

CC BY-NC-ND 4.0

Abstract

The metabolism of 3-deoxy-3-fluoro-D-glucose (3FG) by flight muscle and fat body homogenates of locusts was investigated by initially examining the extent of oxygen consumption with 3FG as substrate. 3FG was not only found to be metabolised by both homogenates at a much slower rate (compared to glucose), but also inhibited oxygen consumption from glucose. The presence of aldose reductase (EC.1.1.1.21, alditol: NADP('+) 1-oxidoreductase), and sorbitol dehydrogenase EC.1.1.1.14, alditol:NAD('+)5-oxidoreductase) enzymes have been shown to be present in the flight muscle of locusts, with the flight muscle enzymes exhibiting a higher affinity for their respective natural substrates compared to the fat body enzymes. Both tissues have been shown to be capable, not only of glycolysing 3FG, but also of synthesising glycogen and trehalose from 3FG, which was evident in the radiochromatographic, quantitative and ('19)F-NMR studies. With glycolysis, 3FG was found to be metabolised as far down as the triose sugars with significant amounts of detritiation and defluorination. Finally, the irreversible toxic effects of 3FG is postulated to be via fluoride release (from a metabolite of 3FG) concomitant to alkylation of triosephosphate isomerase (E.C.5.3.1.1), leading to the reversal of glycolysis, hence, the accumulation of fructose. Also implicated to compound the toxic effects of 3FG is the possible alkylation of the enzyme, trehalase (E.C.3.2.1.21), which also led to the accumulation of fluorotrehalose.Dept. of Chemistry and Biochemistry. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1983 .A322. Source: Dissertation Abstracts International, Volume: 44-03, Section: B, page: 0771. Thesis (Ph.D.)--University of Windsor (Canada), 1983.

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