Title

The role and regulation of cathepsin B during skeletal muscle cell differentiation.

Date of Award

1998

Degree Type

Dissertation

Degree Name

Ph.D.

Department

Biological Sciences

First Advisor

Dufresne, M. J.

Keywords

Biology, Cell.

Rights

CC BY-NC-ND 4.0

Abstract

The results reported in this dissertation provide evidence that the cysteine protease, cathepsin B, is one of the nonmuscle-specific genes expressed in a regulated manner during myogenesis. Unlike muscle-specific enzymes such as creatine phosphokinase (CPK), levels of cathepsin B activity are high in dividing, presumptive myoblasts. As these cells align in linear arrays in preparation for fusion, levels of cathepsin B activity drop dramatically and then increase in a fusion-related manner characteristic of muscle specific enzymes. This current studies appears to be the first to demonstrate a "down-up" pattern of cathepsin B activity over the entire differentiation process in vitro and to provide both indirect and direct evidence for the role of cathepsin B in myogenesis. Source: Dissertation Abstracts International, Volume: 66-02, Section: B, page: 0659. Adviser: Michael J. DuFresne. Thesis (Ph.D.)--University of Windsor (Canada), 1998.