Date of Award
Fidalgo da Silva, Elizabeth
cell cycle, cyclin B1, localization, mitosis, tuberous sclerosis
CC BY-NC-ND 4.0
The cell cycle is a highly dynamic and phasic process controlling cell growth, DNA duplication, and successful division into two daughter cells. Regulation of this process is key to avoiding errors and activation of cell death. Tumour suppressor proteins, such as Tuberin, and cell cycle proteins, such as Cyclin B1 are highly important in co-ordinating adequate cell growth and properly timed cell division, respectively. It is known that mutation, truncation, and misregulation of the Tuberin protein can result in diseases like Tuberous Sclerosis and cancer. This study demonstrated that a clinically relevant Tuberin truncation, S664Δ, increases cellular proliferation and exhibits aberrant localization compared to wildtype Tuberin. Additionally, our study showed that S664Δ is able to bind Cyclin B1, which may derange proper co-ordination of the G2/M transition. Findings here may have clinical implications towards better understanding the progression of disease involving misregulated Tuberin.
Dare-Shih, Jessica Morgan, "IMPLICATIONS OF MITOTIC ONSET REGULATION IN TUBEROUS SCLEROSIS" (2016). Electronic Theses and Dissertations. 5633.