Date of Award

2-3-2016

Degree Type

Thesis

Degree Name

M.Sc.

Department

Biological Sciences

First Advisor

Porter, Lisa

Second Advisor

Fidalgo da Silva, Elizabeth

Keywords

cell cycle, cyclin B1, localization, mitosis, tuberous sclerosis

Rights

CC BY-NC-ND 4.0

Abstract

The cell cycle is a highly dynamic and phasic process controlling cell growth, DNA duplication, and successful division into two daughter cells. Regulation of this process is key to avoiding errors and activation of cell death. Tumour suppressor proteins, such as Tuberin, and cell cycle proteins, such as Cyclin B1 are highly important in co-ordinating adequate cell growth and properly timed cell division, respectively. It is known that mutation, truncation, and misregulation of the Tuberin protein can result in diseases like Tuberous Sclerosis and cancer. This study demonstrated that a clinically relevant Tuberin truncation, S664Δ, increases cellular proliferation and exhibits aberrant localization compared to wildtype Tuberin. Additionally, our study showed that S664Δ is able to bind Cyclin B1, which may derange proper co-ordination of the G2/M transition. Findings here may have clinical implications towards better understanding the progression of disease involving misregulated Tuberin.

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