Date of Award
1-12-2016
Publication Type
Master Thesis
Degree Name
M.H.K.
Department
Kinesiology
Keywords
axon, injury, whiplash
Supervisor
Azar, Nadia
Rights
info:eu-repo/semantics/openAccess
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Abstract
This study investigated the presence of axonal injury within cervical facet joint capsules (FJC) exposed to a high-rate (100 mm/s) tensile stretch. The left C5-C6 FJCs of five anaesthetized goats were subjected to a series of tensile tests in 4 mm increments until rupture (the intact right FJCs served as controls). The FJCs were harvested, fixed in 4% buffered paraformaldehyde, embedded in paraffin, and serially sectioned. FJC sections were immunolabeled for neurofilament light chain (NF-L) and beta-amyloid precursor protein (β-APP). A significantly higher frequency of coupled β-APP/NF-L immunoreactive sections was found in stretched (23.8%) compared to unstretched FJCs (6.3%, p = 0.02). This finding suggests that high-rate tensile stretch is a mechanism for axonal injury in cervical FJCs, and furthers the understanding of axonal injury in the whiplash pain mechanism. The use of the dual immunolabeling, presents a new method for identifying axonal injury in skeletal tissue.
Recommended Citation
Shaw, Christopher Stuart, "Identifying Axonal Injury in Cervical Facet Joint Capsules as a Result of High-Rate Tensile Stretch in an In-vivo Goat Model" (2016). Electronic Theses and Dissertations. 5666.
https://scholar.uwindsor.ca/etd/5666