Title

Novel and optimized polymorphic microsatellite loci for brown bullhead (Ameiurus nebulosus)

Document Type

Article

Publication Date

2010

Publication Title

Conservation Genetics Resources

Volume

2

Issue

1

First Page

211

Last Page

213

DOI

10.1007/s12686-010-9239-9

Keywords

Ameiurus nebulosus; Bullhead; Cross-amplification; diversity; great-lakes; markers; microsatellites

Abstract

Eight novel polymorphic microsatellite markers were developed for brown bullhead (Ameiurus nebulosus). Allelic diversity varied between three and eleven alleles per locus with observed heterozygosity ranging from 0.286 to 0.776 among 49 Detroit River fish, with no evidence of linkage disequilibrium. One locus showed deviation from Hardy-Weinberg equilibrium likely due to null alleles. The new markers amplified in four related catfish species. Five additional primers from channel catfish (Ictalurus punctatus) and yellow bullhead (A. natalis) were optimised for use with brown bullhead.

Comments

This is an accepted manuscript version of an aritcle whose version of record was published in:Conservation Genetics Resources: http://dx.doi.org/10.1007/s12686-010-9239-9