Title

Assessing the impact of palmitic, myristic and lauric acids on hydrogen production from glucose fermentation by mixed anaerobic granular cultures

Document Type

Article

Publication Date

2012

Publication Title

International Journal of Hydrogen Energy

Volume

37

Issue

24

First Page

18761

Last Page

18772

DOI

10.1016/j.ijhydene.2012.09.116

Keywords

bacteria; chain fatty-acids; degradation; enhanced biohydrogen production; Flux balance analysis; Hydrogen; inhibition; linoleic-acid; Long chain fatty acids; metabolism; Methanogenesis; Microbial diversity; Mixed anaerobic cultures; ph; Principal component analysis; sewage-sludge

Abstract

The effects of lauric (LUA), myristic (MA), palmitic (PA), and a mixture of myristic:palmitic (MA:PA) acids on hydrogen (H-2) production from glucose degradation using anaerobic mixed cultures were assessed at 37 degrees C with an initial pH set at 5.0 and 7.131 mM of each acid. The maximum H-2 yield (2.53 +/- 0.18 mol mol(-1) glucose) was observed in cultures treated with PA. A principal component analysis (PCA) of the by-products and the microbial population data sets detected similarities between the controls and PA treated cultures; however, differences were observed between the controls and PA treated cultures in comparison to the MA and LUA treated cultures. The flux balance analysis (FBA) showed that PA decreased the quantity of H-2 consumed via homoacetogenesis compared to the other LCFAs. The control culture was dominated by Thermoanaerovibrio acidaminovorans (60%), Geobacillus sp. and Eubacterium sp. (28%), while Clostridium sp. was less than 1%. Treatment with PA, MA, MA:PA, or LUA increased the H-2 producers (Clostridium sp. and Bacillus sp.) population by approximately 48, 67, 86, and 86%, respectively. Copyright (C) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.

Comments

This is an accepted manuscript version of an aritcle whose version of record was published in:International Journal of Hydrogen Energy: http://dx.doi.org/10.1016/j.ijhydene.2012.09.116