Title

Effect of inhibitors on hydrogen consumption and microbial population dynamics in mixed anaerobic cultures

Document Type

Article

Publication Date

2014

Publication Title

International Journal of Hydrogen Energy

Volume

39

Issue

1

First Page

249

Last Page

257

DOI

10.1016/j.ijhydene.2013.10.084

Keywords

Anaerobic mixed cultures; chain fatty-acids; degradation; enhanced biohydrogen production; fermentation in-vitro; fish oils; Furfural; Hydrogen consumption; linoleic-acid; methane; pretreatment; rumen fermentation; ruminal methanogenesis; Saturated long chain fatty acid; Unsaturated fish oil; Unsaturated long chain fatty acid

Abstract

The impact of different chemical microbial stressors (2-bromoethanesulfonate (BES), furfural, fish oil, lauric acid (LUA) and linoleic acid (LA)) on the inhibition of mesophilic hydrogen (H-2) consumption was examined in this study. Hydrogen consumption half-life values were used to compare the extent of inhibition by the different microbial stressing agents. A statistical analysis of the percent H-2 consumed using Tukey's analysis revealed the following trend: Control > fish oil = linoleic acid (LA (C18:2)) = furfural > BES > lauric acid (LUA (C12:0). The terminal restriction fragment length polymorphism (T-RLFP) results indicated that aceticlastic methanogens (Methanosaeta sp., Methanosarcina sp.) and hydrogenotrophic methanogens (Methanococcus sp.) were inhibited by the different chemical stressing agents. Cultures fed LUA and LA had a high abundance of Clostridium sp., Clostridium propionicum and Propionibacterium acnes. In comparison, BES and furfural fed cultures contained large fractions of Clostridium sp., Eubacteria sp. and Bacteroides sp. while in the fish oil fed cultures, the dominant organism detected was Eubacteria sp. This study indicated that H-2 consumption was affected by the chemical stressing agent concentration. Copyright (C) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.

Comments

This is an accepted manuscript version of an aritcle whose version of record was published in:International Journal of Hydrogen Energy: http://dx.doi.org/10.1016/j.ijhydene.2013.10.084