Document Type

Article

Publication Date

2015

Publication Title

Water

Volume

7

Issue

5

First Page

2275

Last Page

2292

DOI

10.3390/w7052275

Keywords

anaerobic-digestion; anaerobic sequencing batch reactor; cod; competition; electron-donors; fatty-acids; fluidized-bed; gas-lift reactor; methanogens; mine drainage; reducing bacteria; sequencing batch reactor; SO42- ratio; sulfate reducing bacteria; sulfate reduction; waste-water treatment

Abstract

Biological sulfate (SO42-) reduction was examined in anaerobic sequential batch reactors (ASBRs) operated under different hydraulic retention times (HRTs) ranging from 12 to 36 h and COD (Chemical Oxygen Demand)/SO42- ratios of 2.4, 1.6 and 0.8. Competition between SO42- reducing bacteria (SRBs), methane producing archaea (MPAs) and homoacetogens (HACs) was examined in controls and cultures treated with linoleic acid (LA). The ASBR performance was influenced by the COD/SO42- ratio in control cultures with a SO42- reduction of 87% at a COD/SO42- ratio of 0.8. At a 12 h HRT, in both control and LA treated cultures, greater than 75% SO42- removal was observed under all the conditions examined. In control reactors operating at a 36 h HRT, high levels of MPAs belonging to Methanobacteriales and Methanosarcinales were detected; however, in comparison, under low COD/SO42- ratio and with decreasing HRT conditions, a relative increase in SRBs belonging to Desulfovibrio and Desulfatibacillum was observed. Adding 0.5 gL(-1) LA suppressed Methanobacteriales, while increasing the LA concentration to 1 gL(-1) completely suppressed MPAs with a relative increase in SRBs. HACs belonging to Bacteroidetes were observed in the control and in cultures operated at 12 h HRT with a COD/SO42- ratio of 1.6 and fed 0.5 gL(-1) LA; however, with all other LA levels (0.5 and 1.0 gL(-1)) and HRTs (12, 24 and 36 h), HACs were not detected.

Comments

http://dx.doi.org/10.3390/w7052275

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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