Document Type

Article

Publication Date

2013

Publication Title

Biomedical Optics Express

Volume

4

First Page

481

Last Page

489

DOI

10.1364/BOE.4.000481

Keywords

Spectroscopy, Medical optics and biotechnology, Chemometrics, Raman spectroscopy

Abstract

The determination of bacterial identity at the strain level is still a complex and time-consuming endeavor. In this study, visible wavelength spontaneous Raman spectroscopy has been used for the discrimination of four closely related Escherichia coli strains: pathogenic enterohemorrhagic E. coli O157:H7 and non-pathogenic E. coli C, E. coli Hfr K-12, and E. coli HF4714. Raman spectra from 600 to 2000 cm-1 were analyzed with two multivariate chemometric techniques, principal component-discriminant function analysis and partial least squares-discriminant analysis, to determine optimal parameters for the discrimination of pathogenic E. coli from the non-pathogenic strains. Spectral preprocessing techniques such as smoothing with windows of various sizes and differentiation were investigated. The sensitivity and specificity of both techniques was in excess of 95%, determined by external testing of the chemometric models. This study suggests that spontaneous Raman spectroscopy with visible wavelength excitation is potentially useful for the rapid identification and classification of clinically-relevant bacteria at the strain level.

Funding Reference Number

RGPIN/418254-2012

Comments

This work was supported in part by a Natural Sciences and Engineering Research Council of Canada Discovery Grant (RGPIN/418254-2012).

First Published online at http://dx.doi.org/10.1364/BOE.4.000481

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