Type of Proposal

Oral presentation

Start Date

24-3-2015 2:00 PM

End Date

24-3-2015 2:50 PM

Faculty

Faculty of Science

Faculty Sponsor

Dr. Bulent Mutus

Importance of the Project

My research is to follow-up on the findings of a previous student which proposed the presence of a potentially essential conserved lysine residue neighbouring the redox active sites of both a and a' domains of protein disulfide isomerase (PDI). I will be employing various techniques to further study the role of these active site lysine residues (Lys57 and Lys401 of human PDI, specifically).

Existing State of Knowledge

PDI has two redox-active thioredoxin-like domains: a and a', each with a highly conserved -CXXC- active site motif. The mechanistic workings of this motif are well characterized. It has been shown by a Japanese research group that the ability of PDI to catalyze the isomerization or oxidation-reduction of a substrate's disulfide bonds depends not only on the described motif, but also a neighbouring lysine residue found directly C-terminal to such.

Research Question

Is an active site lysine residue, found quite conserved, of catalytic importance to PDI's oxidoreductase activity?

Methodology

Fluorogenic assay to measure enzyme kinetic data.

Ref: Raturi, A., and Mutus, B., 2007, "Characterization of redox state and reductase activity of protein disulfide isomerase under different redox environments using a sensitive fluorescent assay," Free Radical Biol. & Med., v. 43, p. 62-70.

pH-dependency kinetic assays.

Protein acetylation.

Computational molecular dynamics to further study structural aspects of PDI (collaborative work).

Your Findings

Active site lysine mutations have proven detrimental to the catalytic activity of PDI as indicated by appreciable decreases in both both Vmax and catalytic efficiency. Consistent kM values between the wild type and mutant enzymes give indications that the active site conformation is not altered as a result of mutation. Computational analysis should indicate the primary role of active site lysine residues in PDI's oxidoreductase activity.

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Mar 24th, 2:00 PM Mar 24th, 2:50 PM

Investigating the Impact of Active Site Mutations on the Oxidoreductase Activity of Protein Disulfide Isomerase