Title

Exploring the Efficacy of Sage Ethanolic Extract on Lymphoma Cell Lines

Type of Proposal

Oral presentation

Start Date

31-3-2017 9:00 AM

End Date

31-3-2017 10:20 AM

Faculty

Faculty of Science

Faculty Sponsor

Siyaram Pandey

Abstract

Cancer is a persistent and unavoidable occurrence in today’s society. Current approaches to treatment such as chemotherapy and surgery have proven effective in treating many diagnosed patients, but have been shown to cause severe side effects such as neurotoxicity, ototoxicity, numerous organ system disorders, and hematological toxicity. Lymphoma accounts for 3.2% of all cancer-related deaths, and poses a challenge in treatments due to the difficulty of treatment and observed toxicity. Natural health products (NHPs) offer a viable option in cancer treatment and have been shown by previous research to have high anti-cancer efficacy, reduce adverse side-effects, and have low toxicity to lymphoma models used today. In addition, these NHPs have the potential to be selective for cancer cells, preventing adverse effects to normal cells. My objective is to analyze a sage extract, which has been shown to be have a high anti-cancer efficacy, and to analyze the efficacy of these extracts in vitro. Specifically, I plan to do this by assessing the viability, proliferation, apoptosis, and autophagy of treated cancer cells using a variety of assays, and determining the mechanism of action. Furthermore, the extract will be fractionated to identify the biologically active component(s) of sage, which will be used in treatment to analyze treatment compared to normal extract treatments. Preliminary research shows that ethanolic extracts of long pepper and sage are effective in reducing the viability of lymphoma cells in a dose and time dependent manner, and a synergistic benefit has been observed in combination treatments. These results are of significant importantce and have the potential to allow for this natural health product developed into safer anti-cancer agents and possibly brought to market for human use.

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Mar 31st, 9:00 AM Mar 31st, 10:20 AM

Exploring the Efficacy of Sage Ethanolic Extract on Lymphoma Cell Lines

Cancer is a persistent and unavoidable occurrence in today’s society. Current approaches to treatment such as chemotherapy and surgery have proven effective in treating many diagnosed patients, but have been shown to cause severe side effects such as neurotoxicity, ototoxicity, numerous organ system disorders, and hematological toxicity. Lymphoma accounts for 3.2% of all cancer-related deaths, and poses a challenge in treatments due to the difficulty of treatment and observed toxicity. Natural health products (NHPs) offer a viable option in cancer treatment and have been shown by previous research to have high anti-cancer efficacy, reduce adverse side-effects, and have low toxicity to lymphoma models used today. In addition, these NHPs have the potential to be selective for cancer cells, preventing adverse effects to normal cells. My objective is to analyze a sage extract, which has been shown to be have a high anti-cancer efficacy, and to analyze the efficacy of these extracts in vitro. Specifically, I plan to do this by assessing the viability, proliferation, apoptosis, and autophagy of treated cancer cells using a variety of assays, and determining the mechanism of action. Furthermore, the extract will be fractionated to identify the biologically active component(s) of sage, which will be used in treatment to analyze treatment compared to normal extract treatments. Preliminary research shows that ethanolic extracts of long pepper and sage are effective in reducing the viability of lymphoma cells in a dose and time dependent manner, and a synergistic benefit has been observed in combination treatments. These results are of significant importantce and have the potential to allow for this natural health product developed into safer anti-cancer agents and possibly brought to market for human use.