microarray, Pitx3, morpholino, Xenopus laevis, eye, lens, somite, Pax6, segmentation clock, retinoid, mutant
Background: Unexpected phenotypes resulting from morpholino-mediated translational knockdown of Pitx3 in Xenopus laevis required further investigation regarding the genetic networks in which the gene might play a role. Microarray analysis was, therefore, used to assess global transcriptional changes downstream of Pitx3. Results: From the large data set generated, selected candidate genes were confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR) and in situ hybridization. Conclusions: We have identified four genes as likely direct targets of Pitx3 action: Pax6, beta Crystallin-b1 (Crybb1), Hes7.1, and Hes4. Four others show equivocal promise worthy of consideration: Vent2, and Ripply2 (aka Ledgerline or Stripy), eFGF and RXRa. We also describe the expression pattern of additional and novel genes that are Pitx3-sensitive but that are unlikely to be direct targets.
Hooker, L; Smoczer, C; Khosrowshahian, Farhad; and Crawford, Michael J., "Microarray-based identification of Pitx3 targets during Xenopus embryogenesis" (2012). Developmental Dynamics, 241, 9, 1487-1505.