Title
Microinjection manipulations in the elucidation of xenopus brain development
Document Type
Article
Publication Date
2014
Publication Title
Methods in Molecular Biology
Volume
1082
First Page
143
Last Page
154
Abstract
Microinjection has a long and distinguished history in Xenopus and has been used to introduce a surprisingly diverse array of agents into embryos by both intra- and intercellular means. In addition to nuclei, investigators have variously injected peptides, antibodies, biologically active chemicals, lineage markers, mRNA, DNA, morpholinos, and enzymes. While enumerating many of the different microinjection approaches that can be taken, we will focus upon the mechanical operations and options available to introduce mRNA, DNA, and morpholinos intracellularly into early stage embryos for the study of neurogenesis.
DOI
10.1007/978-1-62703-655-9-10
Recommended Citation
Smoczer, Cristine; Hooker, Lara Nicole; Sachani, Saqib; and Crawford, Michael J., "Microinjection manipulations in the elucidation of xenopus brain development" (2014). Methods in Molecular Biology, 1082, 143-154.
https://scholar.uwindsor.ca/biologypub/111