Biochemistry and Cell Biology
regeneration, Xenopus laevis, linib bud, Pitx1 protein, specification
In contrast to the pattern of limb emergence in mammals, chicks, and the newt N. viridescens, embryos such as Xenopus laevis and Eleutherodactylus coqui initiate pelvic limb buds before they develop pectoral ones. We studied the expression of Pitx1 in X. laevis and E. coqui to determine if this paired-like homeodomain transcription factor directs differentiation specifically of the hindlinib, or if it directs the second pair of limbs to form, namely the forelimbs. We also undertook to determine if embryonic expression patterns were recapitulated during the regeneration of an amputated limb bud. Pitx1 is expressed in hindlimbs in both X. laevis and E. coqui, and expression is similar in both developing and regenerating limb buds. Expression in hindlimbs is restricted to regions of proliferating mesenchyme.
Chang, WY; Khosrowshahian, Farhad; Wolanski, Marian; and Marshall, R, "Conservation of Pitx1 expression during amphibian limb morphogenesis" (2006). Biochemistry and Cell Biology, 84, 2, 257-262.