Title

Neutron diffraction from aligned stacks of lipid bilayers using the WAND instrument

Document Type

Article

Publication Date

4-1-2018

Publication Title

Journal of Applied Crystallography

Volume

51

Issue

2

First Page

235

Last Page

241

DOI

10.1107/S1600576718001243

Keywords

deuteration, lipid bilayers, neutron diffraction, relative humidity

Abstract

© Drew Marquardt et al. 2018 Neutron diffraction from aligned stacks of lipid bilayers is examined using the Wide-Angle Neutron Diffractometer (WAND), located at the High Flux Isotope Reactor, Oak Ridge, Tennessee, USA. Data were collected at different levels of hydration and neutron contrast by varying the relative humidity (RH) and H2O/D2O ratio from multi-bilayers of dioleoylphosphatidylcholine and sunflower phosphatidylcholine extract aligned on single-crystal silicon substrates. This work highlights the capabilites of a newly fabricated sample hydration cell, which allows the lipid bilayers to be hydrated with varying H/D ratios from the RH generated by saturated salt solutions, and also demonstrates WAND's capability as an instrument suitable for the study of aligned lipid multi-bilayers.

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