Biochimica et Biophysica Acta - Biomembranes
KL 4, Lung surfactant, Membranes
We report on the orientation and location of synthetic pulmonary surfactant peptide KL4, (KLLLL)4K, in model lipid membranes. The partitioning depths of selectively deuterated leucine residues within KL4 were determined in DPPC:POPG (4:1) and POPC:POPG (4:1) bilayers by oriented neutron diffraction. These measurements were combined with an NMR-generated model of the peptide structure to determine the orientation and partitioning of the peptide at the lipid–water interface. The results demonstrate KL4 adopting an orientation that interacts with a single membrane leaflet. These observations are consistent with past 2H NMR and EPR studies (Antharam et al., 2009; Turner et al., 2014).
Marquardt, Drew; van Oosten, Brad; Dziura, Maksymilian; Long, Joanna R.; and Harroun, Thad A.. (2022). The interaction and orientation of Peptide KL4 in model membranes. Biochimica et Biophysica Acta - Biomembranes, 1864 (7).