Methods in Molecular Biology
Contrast-matching, Domains, Lipid rafts, Lipid vesicles, Phase separation, Small angle neutron scattering
Lipid domains in model membranes are routinely studied to provide insight into the physical interactions that drive raft formation in cellular membranes. Using small angle neutron scattering, contrast-matching techniques enable the detection of lipid domains ranging from tens to hundreds of nanometers which are not accessible to other techniques without the use of extrinsic probes. Here, we describe a probe-free experimental approach and model-free analysis to identify lipid domains in freely floating vesicles of ternary phase separating lipid mixtures.
DiPasquale, Mitchell; Nguyen, Michael H.L.; Castillo, Stuart R.; Heberle, Frederick A.; and Marquardt, Drew. (2022). Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering. Methods in Molecular Biology, 2402, 163-177.