Ion-spray mass spectrometry of marine neurotoxins.
Rapid communications in mass spectrometry : RCM
domoic acid, drug derivative, kainic acid, marine toxin, neurotoxin, saxitoxin, tetrodotoxin, article, mass spectrometry, methodology, Kainic Acid, Marine Toxins, Neurotoxins, Saxitoxin, Spectrum Analysis, Mass, Tetrodotoxin
Ion-spray mass spectrometry was investigated for the analysis of three marine neurotoxins: domoic acid, saxitoxin and tetrodotoxin. All three compounds gave positive-ion spectra with abundant ions of protonated molecules and no significant fragmentation. Domoic acid gave a negative-ion spectrum with a strong [M-H]- ion. Tandem mass spectrometry provided useful fragment-ion spectra for all compounds. Detection limits for flow injection analyses with selected-ion monitoring were determined to be 30 pg for saxitoxin, 100 pg for domoic acid and 200 pg for tetrodotoxin. Combining liquid chromatography with ion-spray mass spectrometry allowed the determination of domoic acid and some of its isomers in toxic shellfish tissue extracts.
Quilliam, M.A.; Thomson, B.A.; Scott, G.J.; and Siu, K.W. Michael. (1989). Ion-spray mass spectrometry of marine neurotoxins.. Rapid communications in mass spectrometry : RCM, 3 (5), 145-150.