Date of Award


Publication Type

Master Thesis

Degree Name



Civil and Environmental Engineering

First Advisor

Bewtra, J. K.,


Engineering, Environmental.




The use of sulfate reducing bacteria, SRB, showed promising results in removing heavy metals partly by sulfides precipitation and partly by biosorption, simultaneously, in upflow anaerobic fixed film reactors, UAFFRs, for different hydraulic retention times, HRTs. The SRB were capable to grow optimally under certain growth conditions for each HRT by utilizing lactate as organic carbon source. The mathematical relations for design of UAFFR for optimum metal removal for different hydraulic retention times, HRTs, have been developed. The first phase (Phase I) of this research studied the occupation and productivity of SRB in the entire reactor height for different hydraulic retention times, HRTs. They were found in almost constant concentrations in the entire height and different for different HRTS, which proves that growth of SRB was achieved in the lowest region (upto 0.3 m). Also, the sulfide concentration were found different at different heights and different for different HRTs, which concludes that the production of sulfide was attained all along the reactor height. A mathematical equation been developed to correlate the sulfide productivity to different reactor heights. In the second phase (Phase II), the performance of SRB in removing heavy metal at different HRTs was studied. Copper was applied in different concentrations, for each HRT, until complete failure of the reactor. The 18 h HRT reactor was capable to remove 90% of over 200 mg/L influent copper, 85% of over 300 mg/L influent copper and 80% of over 400 mg/L influent copper. Whereas, the 9 h HRT reactor removed 87% of over 200 mg/L influent copper, 75% of over 300 mg/L influent copper and 67% of over 400 mg/L influent copper.Dept. of Environmental Engineering. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1998 .S54. Source: Masters Abstracts International, Volume: 39-02, page: 0572. Adviser: J. K. Bewtra. Thesis (M.A.Sc.)--University of Windsor (Canada), 1998.