Date of Award


Publication Type

Master Thesis

Degree Name



Civil and Environmental Engineering

First Advisor

Bewtra, J. K.,


Engineering, Civil.




The effects of hydraulic retention time and attachment media on the rate of microbial sulfate reduction with lactate as carbon and energy source were investigated for sulfate reducing bacteria, SRB. The lactate to sulfate ratio was maintained below 2 for proper SRB activity. The Th.O.D.:N:P was kept at 100:5:1. The presence of cations, such as Na+ and Mg 2+, below non-toxic concentrations was important to avoid any growth inhibition of SRB. Batch anaerobic reactors were used in order to determine the stoichiometry of the biochemical reactions. Results indicated that 70% of the lactate fed was bio-oxidized and 30% was bio-synthesized, which showed that the energy production reaction predominated over the synthesis reaction in non-packed reactors. Continuous mode operation of three upflow anaerobic suspended growth reactors yielded effluent sulfide concentrations as high as 190 mg S/L at the optimum conditions of 20 h of HRT, 1.5 m/d of upflow velocity and 6 kg/d/m 3 of OLR, regardless of the presence of attachment media in the reactor. (Abstract shortened by UMI.)Dept. of Civil and Environmental Engineering. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis2001 .P65. Source: Masters Abstracts International, Volume: 40-03, page: 0749. Adviser: J. K. Bewtra. Thesis (M.A.Sc.)--University of Windsor (Canada), 2001.