Date of Award
1994
Publication Type
Doctoral Thesis
Degree Name
Ph.D.
Department
Chemistry and Biochemistry
Keywords
Chemistry, Biochemistry.
Supervisor
Adeli, K.
Rights
info:eu-repo/semantics/openAccess
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Abstract
Faulty regulation of various apolipoproteins by the human liver may be an important contributor in the development of coronary heart disease. The factors that regulate hepatic apolipoprotein production are largely unknown. In cell culture, insulin appeared to reduce the secretion of apolipoprotein B, whereas thyroid hormone stimulated apolipoprotein B secretion. Both of these effects were found to be dose-dependent. In order to understand the mechanisms underlying the regulation of hepatic apolipoprotein B secretion, a cell-free translation system derived from a human hepatoma cell-line, HepG2, was developed to investigate the rate of apolipoprotein B synthesis. Extracts of HepG2 cells were found to have high in vitro protein synthesizing activity, and were shown to efficiently synthesize in vitro a number of liver specific proteins, including the unusually large apolipoprotein B molecule. This in vitro system along with whole-cell pulse labeling experiments were used to study the effects of insulin and thyroid hormone on apolipoproteins B, E and A-I synthesis and secretion in HepG2 cells. Source: Dissertation Abstracts International, Volume: 56-01, Section: B, page: 0226. Adviser: Khosrow Adeli. Thesis (Ph.D.)--University of Windsor (Canada), 1994.
Recommended Citation
Theriault, Andre G., "Hormonal regulation of apolipoproteins synthesis and secretion in human hepatocytes." (1994). Electronic Theses and Dissertations. 2314.
https://scholar.uwindsor.ca/etd/2314