Date of Award


Publication Type

Master Thesis

Degree Name



Civil and Environmental Engineering


Engineering, Environmental.




Fermentative hydrogen production from biomass using mixed anaerobic cultures has a greater potential to be developed as a practical biohydrogen system than systems utilizing pure cultures. To optimize hydrogen production, it is important to inhibit hydrogen consumers during glucose fermentation. Long chain fatty acids (LCFAs) are inhibitors of aceticlastic methanogenic bacteria and these fatty acids could act as hydrogenotroph methanogenic inhibitor in fermentative hydrogen production. Batch studies were conducted to assess the effects of two C18 LCFAs on microbial hydrogen production from glucose under mesophilic conditions. Experiments were conducted using different concentrations of linoleic acid (LA) and oleic acid (OA) at 37+/-1°C. The effects of initial pH in the presence of two C18 LCFAs on hydrogen production were assessed by controlling the initial pH. Glucose was re-injected on day 4 or day 5 to examine the combined effect of LCFA and volatile fatty acids (VFAs) on hydrogen production and also the inhibition time dependence. (Abstract shortened by UMI.)Dept. of Civil and Environmental Engineering. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis2005 .C46. Source: Masters Abstracts International, Volume: 44-03, page: 1471. Thesis (M.A.Sc.)--University of Windsor (Canada), 2005.