Date of Award


Publication Type

Master Thesis

Degree Name



Civil and Environmental Engineering

First Advisor

Taylor, K. E.,


Engineering, Civil.



Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.


The aim of this study was to observe the kinetic behavior of the co-substrate oxygen in the laccase-catalyzed reaction of cresols and phenol. Under optimum pH, enzyme concentration, and with different concentrations of oxygen, cresol and phenol, the consumption rates of oxygen were recorded at fixed temperature and studied to find out the effects of oxygen in the reaction. It was found that, with increasing cresol and phenol concentrations, initial oxygen consumption velocities increased for both laccase SP850- and laccase SP504-catalyzed reactions. However, oxygen consumption velocities did not always keep increasing with increasing oxygen concentrations for both laccase SP850 and laccase SP504. For laccase SP850, different cresols had different behaviors with varying oxygen concentrations. The initial oxygen consumption velocities were optimal at oxygen concentrations below 8.0 mg/L for m- and p-cresol. But for o-cresol, the initial oxygen consumption kept increasing with increase in oxygen concentration. The reaction between phenol and oxygen was slow compared to the reaction between cresols and oxygen. The initial oxygen consumption velocities at different concentrations of phenol and oxygen were almost the same. (Abstract shortened by UMI.)Dept. of Civil and Environmental Engineering. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis2001 .W36. Source: Masters Abstracts International, Volume: 40-03, page: 0750. Advisers: K. E. Taylor; Nihar Biswas. Thesis (M.A.Sc.)--University of Windsor (Canada), 2001.