Date of Award


Publication Type

Master Thesis

Degree Name



Civil and Environmental Engineering


Engineering, Environmental.


Bewtra, J. K.,




Soybean peroxidase (SBP) enzyme catalyzes the oxidation of various aqueous aromatic compounds in the presence of hydrogen peroxide to form polymers, which are readily precipitated from solution. The enzyme used was extracted from soybean hulls and is called "crude soybean peroxidase". Experiments were conducted to develop a suitable protocol for extracting enzyme from soybean hulls and pertinent characteristics of the crude SBP enzyme were determined. These include the activity, thermostability, phenol content and the hydrogen peroxide demand of the crude enzyme. Experiments were also conducted in batch reactors to investigate the efficiency of crude SBP in removing cresols from unbuffered synthetic wastewater. The optimum conditions to achieve at least 95% removal of the cresols were determined for the following parameters: pH, SBP dose in the presence and absence of PEG (polyethylene glycol), hydrogen peroxide to substrate ratio and PEG dose. (Abstract shortened by UMI.)Dept. of Civil and Environmental Engineering. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1999 .B57. Source: Masters Abstracts International, Volume: 40-03, page: 0762. Advisers: J. K. Bewtra; K. E. Taylor. Thesis (M.A.Sc.)--University of Windsor (Canada), 1999.