Date of Award


Publication Type

Master Thesis

Degree Name



Civil and Environmental Engineering

First Advisor

Bewtra, J. K.,


Engineering, Sanitary and Municipal.




It has been observed that horseradish peroxidase (HRP) can be used to remove phenol and its derivatives from wastewater. However, the large amount of HRP needed has limited its practical application. It has been reported recently that additives like polyethylene glycol (PEG) and gelatin can greatly reduce the amount of enzyme required for polymerization of phenolic compounds in high concentrations. Experiments were carried out to investigate the effect of additives on the HRP catalyzed removal of phenol derivatives at lower concentrations. Phenol derivatives studied included chlorinated phenols and methyl phenols. The results showed that there was a wide pH range, usually 6 to 8, for optimal removal of phenol derivatives. The optimum pH is mostly neutral except for 2-chlorophenol which had optimum pH of 5. Polyethylene glycol can reduce the amount of HRP needed for 95 percent or more removal of phenol derivatives from 1/30 to 1/130 and consequently, the turnovers were increased 30 to 130 times. The minimum PEG dose at a phenolic concentration of 1 mM (about 100 mg/L) varied from 30 to 100 mg/L, depending on the nature of phenolic compound. Extra PEG did not improve its effect. The time needed for the completion of polymerization varied from 1 to 3 hours at the minimum HRP and minimum PEG dose condition. For most of the phenol derivatives investigated, the optimum molar ratio of hydrogen peroxide to phenol derivatives was around 1. Coprecipitation can occur after adding additive.Dept. of Civil and Environmental Engineering. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1993 .W995. Source: Masters Abstracts International, Volume: 32-02, page: 0714. Adviser: J. K. Bewtra. Thesis (M.A.Sc.)--University of Windsor (Canada), 1993.