Date of Award


Publication Type

Master Thesis

Degree Name



Chemistry and Biochemistry


Chemistry, Biochemistry.




Nitric Oxide is an important messenger and mediator in biological systems. Due to its short half life, S-nitrosothiols (RSNOs) have been suggested to be one of the important biomolecules for the storage and transport of NO. RSNOs also exhibit NO-like bioactivities. In the present study, we focus on the study of some aspects of cell biology of lipid S-nitrosothiols. In the first part of the study, we focus on the comparison of the cellular uptake of native LDL and chemically modified (homocysteinylation and S-nitrosation) LDL by Normal Human Fibroblasts (NHFs). Here, S-nitroso homocysteinylated LDL (LDL-SNO) was synthesized through the reaction of homocysteinylated LDL (LDL-SH) and S-nitrosocysteine. In the second part of the study, we focus on the comparison of the role of lipid S-nitrosothiols in apoptosis under light and dark conditions. Here, S-nitroso-octadecanethiol, a novel and lipophilic S-nitrosothiol, was synthesized to explore its apoptotic role in the SK-MEL-28 human melanoma cells under dark and light conditions. The present study demonstrated that S-nitroso-octadecanethiol could induce apoptosis in SK-MEL-28 human melanoma cells. (Abstract shortened by UMI.) Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis2005 .W363. Source: Masters Abstracts International, Volume: 44-03, page: 1371. Thesis (M.Sc.)--University of Windsor (Canada), 2005.