Date of Award


Publication Type

Master Thesis

Degree Name



Chemistry and Biochemistry

First Advisor

Mutus, Bulent,


Chemistry, Biochemistry.




The role of nitric oxide (NO) in biological systems is becoming increasingly important. Initially, the effect of NO on the transport of L-arginine into human platelets was studied. NO is known to have an effect on this system, and platelets have been shown to possess an isoform of the enzyme nitric oxide synthase. Two NO donors (S-nitrosoglutathione and S-nitroso-N-acetyl penicillamine), a source of NO$\sp{+}$ and a solution of authentic NO were used to measure the effect of NO on the L-arginine transport system. The toxicity of nitric oxide can be due to its direct effects, or more likely through higher oxidation products such as peroxynitrite ($\sp{-}$OONO). There is evidence for peroxynitrite production in vivo, and this has been linked to an assortment of pathological conditions. An indirect method of peroxynitrite detection is the formation nitrotyrosine. We have found evidence for nitrotyrosine formation in platelet proteins from patients suffering from prolonged hyperglycemia. Calmodulin is one of the proteins implicated in this peroxynitrite modification. The effect of peroxynitrite on the enzyme glutathione S-transferase was also studied. (Abstract shortened by UMI.)Dept. of Chemistry and Biochemistry. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1997 .H68. Source: Masters Abstracts International, Volume: 37-01, page: 0263. Adviser: Bulent Mutus. Thesis (M.Sc.)--University of Windsor (Canada), 1997.