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The heat shock response is an inherent process found in both eukaryotic and prokaryotic organisms. This response has never been investigated throughout dormancy and germination of Dictyostelium spores. Semi-quantitative analysis revealed hsp70, the main heat shock protein found to be expressed following heat shock at 30°C, to be extremely abundant in dormant spores of mutants aca-[PKA-C], RegA- and SG1. A stage specific dependence for hsp70 was observed throughout development of Ax3, particularly during late aggregation. An overall net decrease of greater than 50% occurred, however, from commencement into development to the end of fruiting. Germination studies revealed the heat shock response to have exhibited the greatest effect from germinating spores of the premature maturation mutant SG2. A 40% overall net increase in hsp70 was evident following heat-induced germination, in comparison to an overall decline of 30% observed from auto-induced germination. (Abstract shortened by UMI.) Source: Masters Abstracts International, Volume: 39-02, page: 0439. Adviser: D. A. Cotter. Thesis (M.Sc.)--University of Windsor (Canada), 2000.
Cervi, David Nicholas., "The molecular chaperone (hsp70) and its potential roles during dormancy and germination of Dictyostelium discoideum spores; correlation with action tyrosine phosphorylation." (2000). Electronic Theses and Dissertations. 4109.