Date of Award
Chemistry and Biochemistry
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1. The structure of the peptide thymosin $\alpha\sb1$ has been investigated by circular dichroism and one- and two-dimensional nuclear magnetic resonance spectroscopy. Thymosin $\alpha\sb1$ is a highly acidic peptide composed of 28 amino acid residues. Through the use of circular dichroism and two-dimensional nuclear magnetic resonance techniques, the structure of thymosin $\alpha\sb1$ in 30% (v/v) deuterated 2,2,2-trifluoroethanol has been solved. Thymosin $\alpha\sb1$ contains an $\alpha$-helix extending from residue 16 to 26 and a turn between residues 5 and 8. Thymosin $\alpha\sb1$ has been shown to be a potent activator of skeletal muscle myosin light chain kinase, a well known calmodulin-dependent enzyme. Using the solution structures of thymosin $\alpha\sb1$ and the calmodulin binding domain of skeletal muscle myosin light chain kinase, computer modelling suggests that electrostatic interactions comprise the major interacting force between these two peptides. 2. The binding of the proposed transition state analog, uridine vanadate to ribonuclease A has been investigated by one- and two-dimensional nuclear magnetic resonance spectroscopy. Analysis of the homonuclear nuclear Overhauser end exchange spectroscopy spectrum of the uridine vanadate/ribonuclease A complex exhibits cross peaks between the H$\varepsilon$1 proton of histidine 12 of RNase A and both the C$\sb6$H and C$\sb5$H protons of uridine vanadate. No cross peaks were observed between the H$\varepsilon$1 proton of histidine 119 of ribonuclease A and the C$\sb6$H and C$\sb5$H protons of uridine vanadate. However, the distances calculated from the crystallographic structure show that the H$\varepsilon$1 proton of histidine 119 is closer to the C$\sb6$H and C$\sb5$H protons of uridine vanadate, than is the H$\varepsilon$1 proton of histidine 12. These results suggests that there is a significant difference in the position of the histidine 119 side chain in the crystallographic and solution structures of the uridine vanadate/ribonuclease A complex.Dept. of Chemistry and Biochemistry. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1994 .V43. Source: Dissertation Abstracts International, Volume: 56-11, Section: B, page: 6099. Adviser: Lana Lee. Thesis (Ph.D.)--University of Windsor (Canada), 1995.
Veenstra, Timothy Daniel., "Two-dimensional nuclear magnetic resonance studies of thymosin-alpha(1) (a myosin light chain kinase activating peptide), and ribonuclease a in the presence of uridine vanadate." (1995). Electronic Theses and Dissertations. 4441.