Date of Award

9-13-2019

Publication Type

Master Thesis

Degree Name

M.A.Sc.

Department

Civil and Environmental Engineering

First Advisor

Biswas, N.

Second Advisor

Taylor, K.E.

Keywords

Bromoxynil, Enzymatic treatment, Ioxynil, Mass spectrometry, Soybean peroxidase

Rights

info:eu-repo/semantics/openAccess

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Abstract

Widely applied pesticides have been detected in water bodies, which threatens the environment and non-target life. Thus, a promising treatment method, soybean peroxidase (SBP)-catalyzed process was studied to remove two halogenated benzonitrile pesticides Bromoxynil and Ioxynil through enzymatic oxidation from synthetic wastewater. SBP can be extracted from soybean hulls, a by-product of the soybean industry. First, the experiments studied the viability of SBP-catalyzed removal on these two compounds, then the operational parameters including pH, the molar ratio between hydrogen peroxide and substrate and minimum effective enzyme concentration were optimized. The first-order rate constant and half-life of each substrate were also determined under the established optimum conditions. The results demonstrated SBP is robust enzyme to achieve more than 95% removal efficiency for both compounds. In addition, the possible oligomeric products after enzymatic treatment were characterized by mass spectrometry and both dehalogenation and hydroxylation were observed after the reaction.

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