Date of Award
Chemistry and Biochemistry
Keith E. Taylor
Health and environmental sciences, Pure sciences, Aromatic compounds, Laccase, Wastewater
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Laccase SP 504 from Trametes villosa, a fungal laccase, was investigated for its capacity to catalyze the oxidative polymerization of hazardous aromatic pollutants (phenol, o-, m-, p-cresol, aniline, o-, m-, p-toluidine) in the presence of O2. Experiments were conducted to determine the optimum pH, minimum enzyme concentration for ≥ 95% conversion of substrate, minimum PEG concentration for optimum effect (if applicable), and the effect of PEG molecular weight on removal efficiencies. Other factors investigated were: the fate of PEG in the reactor, removal over a 3 h reaction period, activity over 3 h reaction period, and the effect of reducing anions and halides in p-cresol conversion. For the three cresol isomers a preliminary kinetic study was done with and without PEG. Lastly the effectiveness of laccase in the removal of phenol in 5 refinery samples was compared to the removal of phenol in synthetic wastewater.
Steevensz, Aaron, "Laccase-catalyzed removal of various aromatic compounds from synthetic and refinery wasterwater" (2008). Electronic Theses and Dissertations. 8068.