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WDR1 is an actin binding protein which promotes actin cytoskeletal rearrangement. Our lab has discovered a truncated isoform of mammalian WDR1, which lacks exons 3-5 (WDRΔ35). The function of both WDR1 and WDRΔ35 has not been characterized. The role of WDR1 and WDRΔ35 in neurite extension was examined using qRT-PCR. The WDR1 RNA levels increased during neurite extension in PC12 cells whereas WDRΔ35 RNA levels decreased. Similarity, the overexpression of WDR1 in PC12 cells increased neurite extension whereas WDRΔ35 overexpression decreased neurite number and length. qRT-PCR was also utilized to examine WDR1 and WDRΔ35 RNA levels during mouse brain development. The WDR1 levels decreased in the CNS tissues in adult mice in comparison to earlier stages. The WDR1 levels were 10-15 fold higher in the various tissues throughout development in comparison to WDRΔ35. These findings implicate an important role for WDR1 and WDRΔ35 in neurite extension processes.
Tousignant, Renée N., "WDR1 and its isoform alter neurite extension in PC12 cells" (2009). Electronic Theses and Dissertations. 8257.